Identification of an Arabidopsis mitochondrial succinate-fumarate translocator

FEBS Lett. 2003 Jan 16;534(1-3):87-92. doi: 10.1016/s0014-5793(02)03782-1.

Abstract

Complementation of a yeast acr1 mutant carrying a deletion of the succinate/fumarate carrier gene enabled functional identification of a mitochondrial succinate translocator from Arabidopsis thaliana (AtmSFC1). Thus complementation of yeast mutants is applicable also for identification and characterization of organellar transporters. Reverse transcription polymerase chain reaction and promoter-GUS fusion showed expression of AtmSFC1 in 2 day old dark grown seedlings, which declined in cotyledons during further development, consistent with a role in export of fumarate for gluconeogenesis during lipid mobilization at early germination of Arabidopsis seeds. In mature plants, expression was found in developing and germinating pollen, suggesting a role in ethanolic fermentation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / genetics
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / genetics*
  • Arabidopsis Proteins / metabolism*
  • Basic-Leucine Zipper Transcription Factors
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism*
  • Cloning, Molecular / methods
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Fumarates / metabolism
  • Gene Expression Regulation, Plant
  • Genetic Complementation Test
  • Mitochondria / metabolism*
  • Mitochondrial Proteins / genetics*
  • Mitochondrial Proteins / metabolism*
  • Molecular Sequence Data
  • Mutation
  • Phylogeny
  • Plant Leaves / genetics
  • Plant Leaves / metabolism
  • Promoter Regions, Genetic
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism
  • Saccharomyces cerevisiae Proteins*
  • Seeds / genetics
  • Seeds / metabolism
  • Succinic Acid / metabolism
  • Yeasts / genetics

Substances

  • Arabidopsis Proteins
  • Basic-Leucine Zipper Transcription Factors
  • Carrier Proteins
  • DNA-Binding Proteins
  • Fumarates
  • Mitochondrial Proteins
  • Recombinant Fusion Proteins
  • Repressor Proteins
  • SKO1 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • fumaric acid
  • Succinic Acid