Notch-1 induction, a novel activity of SV40 required for growth of SV40-transformed human mesothelial cells

Oncogene. 2003 Jan 9;22(1):81-9. doi: 10.1038/sj.onc.1206097.


We show that SV40 infection of human mesothelial cells directly causes overexpression of Notch-1, a key cell regulatory gene. Notch-1 induction is achieved at the transcriptional level and requires both the SV40 large T-antigen and the small t-antigen. Notch-1 upregulation is maintained in SV40-transformed human mesothelial clones and in SV40-positive mesotheliomas and derived cell lines. Activation of Notch-1 promotes cell cycle progression and it is required for the growth of SV40-transformed mesothelial cells. Our finding is relevant to the process of SV40-mediated human cell transformation, an effect that cannot be accounted for solely by SV40-Tag inhibition of Rb and p53.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Line, Transformed
  • Epithelium / metabolism*
  • Humans
  • Membrane Proteins / biosynthesis*
  • Membrane Proteins / genetics
  • Membrane Proteins / physiology
  • Mesothelioma / metabolism
  • Mesothelioma / pathology
  • Mesothelioma / virology
  • RNA, Messenger / genetics
  • Receptor, Notch1
  • Receptors, Cell Surface*
  • Simian virus 40 / physiology*
  • Transcription Factors*
  • Up-Regulation


  • Membrane Proteins
  • NOTCH1 protein, human
  • RNA, Messenger
  • Receptor, Notch1
  • Receptors, Cell Surface
  • Transcription Factors