Predictive value of serum thymidine kinase level for Ig-V mutational status in B-CLL

Leukemia. 2003 Jan;17(1):133-7. doi: 10.1038/sj.leu.2402780.


In B-CLL IgV(H) genes mutational status is a major prognostic factor. Since sequencing of IgV(H) genes is not available in most laboratories, an easily performed surrogate assay is desirable. To identify the best surrogate assay, and to better discriminate prognostic subgroups we analyzed clinical and biological data from 58 typical CLL cases. A higher serum thymidine kinase level (>15 U/l) proved to be a strong predictor of mutational status, and the only independent one among the studied parameters. To further identify prognostic subgroups, cluster analysis was employed on 38 cases on which all data were available, which segregated two groups including 25 and 13 patients, respectively. These two clusters differed by their proliferative potential and appeared to discriminate patients with very different clinical course and outcome. s-TK was strikingly different among these two clusters, suggesting that s-TK level could be used routinely to identify patients at risk of progression.

MeSH terms

  • Aged
  • Antigens, CD / immunology
  • B-Lymphocytes / immunology*
  • Biomarkers, Tumor*
  • Cell Cycle Proteins / metabolism
  • Cyclin D2
  • Cyclin-Dependent Kinase Inhibitor p27
  • Cyclins / metabolism
  • Disease Progression
  • Female
  • Genes, Immunoglobulin / genetics*
  • Humans
  • Immunoglobulin Variable Region / genetics*
  • Immunophenotyping
  • L-Lactate Dehydrogenase / metabolism
  • Leukemia, Lymphocytic, Chronic, B-Cell / diagnosis
  • Leukemia, Lymphocytic, Chronic, B-Cell / genetics*
  • Leukemia, Lymphocytic, Chronic, B-Cell / immunology*
  • Male
  • Middle Aged
  • Mutation*
  • Neoplasm Staging
  • Predictive Value of Tests
  • Prognosis
  • Survival Rate
  • Thymidine Kinase / blood*
  • Tumor Suppressor Proteins / metabolism
  • beta 2-Microglobulin / metabolism


  • Antigens, CD
  • Biomarkers, Tumor
  • CCND2 protein, human
  • Cell Cycle Proteins
  • Cyclin D2
  • Cyclins
  • Immunoglobulin Variable Region
  • Tumor Suppressor Proteins
  • beta 2-Microglobulin
  • Cyclin-Dependent Kinase Inhibitor p27
  • L-Lactate Dehydrogenase
  • Thymidine Kinase