Characterization of phosphodiesterase 3 in human malignant melanoma cell line

Anticancer Res. Nov-Dec 2002;22(6A):3171-4.

Abstract

Little is known concerning the expression, distribution and function of phosphodiesterase (PDE) 3s in malignant tumor cells, including human malignant melanoma HMG and osteosarcoma HOSM-1 cells. PDE3 activity was detected in homogenates of HMG cells; however, much less activity was found in HOSM-1 cells. In HMG cells, most of the PDE3 activity was in the particulate fraction. PDE3A and 3B mRNAs were detected by RT-PCR in RNA from HMG cells only. The nucleotide sequences of the fragments were identical to those of human PDE3A and 3B. The PDE3-specific inhibitors, trequinsin and cilostamide, did not inhibit the proliferation of HMG or HOSM-1 cells. Although two PDE3 isoforms may be expressed in human malignant melanoma cells, their functional importance is not known.

MeSH terms

  • 3',5'-Cyclic-AMP Phosphodiesterases / antagonists & inhibitors
  • 3',5'-Cyclic-AMP Phosphodiesterases / biosynthesis
  • 3',5'-Cyclic-AMP Phosphodiesterases / genetics
  • 3',5'-Cyclic-AMP Phosphodiesterases / metabolism*
  • Bone Neoplasms / enzymology
  • Cell Division / physiology
  • Cyclic AMP / metabolism
  • Cyclic Nucleotide Phosphodiesterases, Type 3
  • Humans
  • Isoenzymes / biosynthesis
  • Isoenzymes / genetics
  • Isoenzymes / metabolism
  • Isoquinolines / pharmacology
  • Melanoma / enzymology*
  • Osteosarcoma / enzymology
  • Phosphodiesterase Inhibitors / pharmacology
  • Quinolones / pharmacology
  • Tetrahydroisoquinolines*
  • Tritium
  • Tumor Cells, Cultured

Substances

  • Isoenzymes
  • Isoquinolines
  • Phosphodiesterase Inhibitors
  • Quinolones
  • Tetrahydroisoquinolines
  • Tritium
  • cilostamide
  • trequinsin
  • Cyclic AMP
  • 3',5'-Cyclic-AMP Phosphodiesterases
  • Cyclic Nucleotide Phosphodiesterases, Type 3
  • PDE3A protein, human