Isolation and characterization of four cell wall purple acid phosphatase genes from tobacco cells

Biochim Biophys Acta. 2003 Jan 27;1625(2):134-40. doi: 10.1016/s0167-4781(02)00599-7.

Abstract

Four full-length cDNAs were isolated from a cDNA library prepared from tobacco cultured cells and designated NtPAP4, NtPAP12, NtPAP19 and NtPAP21, which could correspond to purple acid phosphatase (PAP). Levels of both NtPAP12 and NtPAP21 mRNA in the protoplasts immediately increased after the protoplasts were transferred to a medium for cell wall regeneration, and the accumulation of the mRNA was correlated with cell wall regeneration for 3 h. It is likely that the NtPAP12 and NtPAP21 gene products are wall-bound PAPs at the early stage of regenerating walls in tobacco protoplasts.

Publication types

  • Comparative Study

MeSH terms

  • Acid Phosphatase / chemistry
  • Acid Phosphatase / genetics*
  • Amino Acid Sequence
  • Cell Wall / enzymology*
  • Cells, Cultured
  • DNA, Complementary / isolation & purification
  • Gene Expression Regulation, Plant
  • Genes, Plant*
  • Glycoproteins / chemistry
  • Glycoproteins / genetics*
  • Isoenzymes / genetics
  • Molecular Sequence Data
  • Peptide Fragments / chemistry
  • Phylogeny
  • Protoplasts / metabolism
  • RNA, Messenger / analysis
  • Sequence Alignment
  • Time Factors
  • Tobacco / genetics*

Substances

  • DNA, Complementary
  • Glycoproteins
  • Isoenzymes
  • Peptide Fragments
  • RNA, Messenger
  • purple acid phosphatase
  • Acid Phosphatase