Single multiplex polymerase chain reaction to detect diverse loci associated with diarrheagenic Escherichia coli

Emerg Infect Dis. 2003 Jan;9(1):127-31. doi: 10.3201/eid0901.010507.


We developed and tested a single multiplex polymerase chain reaction (PCR) that detects enterotoxigenic, enteropathogenic, enteroinvasive, and Shiga toxin-producing Escherichia coli. This PCR is specific, sensitive, and rapid in detecting target isolates in stool and food. Because of its simplicity, economy, and efficiency, this protocol warrants further evaluation in large, prospective studies of polymicrobial substances.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Primers
  • Diarrhea / microbiology*
  • Escherichia coli / genetics
  • Escherichia coli / pathogenicity*
  • Escherichia coli Infections / microbiology
  • Escherichia coli Proteins / genetics*
  • Escherichia coli Proteins / metabolism
  • Feces / microbiology
  • Food Microbiology
  • Humans
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity


  • DNA Primers
  • Escherichia coli Proteins