Effect of high-fat diet on sarcoplasmic reticulum Ca(++)-ATPase activity in different types of rat skeletal muscle

Rocz Akad Med Bialymst. 2002;47:31-9.


The aim of the present study was to examine the effect of a high-fat diet on the Ca(2+)-ATPase activity in the sarcoplasmic reticulum of the flexor digitorum longus (fast-twitch, glycolytic muscle) and the soleus (slow-twitch, oxidative muscle). The enzyme activity in the extensor digitorum longus was several-fold higher than in the soleus. The high-fat diet increased the enzyme activity by 22% (p < 0.05) in the soleus but it had no effect in the flexor digitorum longus. It is concluded that a high-fat diet increases the activity of only one isoform of the enzyme, namely the one which is present in the slow-twitch oxidative fibers.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium-Transporting ATPases / analysis
  • Calcium-Transporting ATPases / metabolism*
  • Culture Techniques
  • Diet*
  • Dietary Fats / administration & dosage*
  • Disease Models, Animal
  • Enzyme Activation
  • Male
  • Muscle, Skeletal / enzymology*
  • Muscle, Skeletal / physiopathology
  • Probability
  • Random Allocation
  • Rats
  • Rats, Sprague-Dawley
  • Reference Values
  • Sarcoplasmic Reticulum / enzymology*
  • Sensitivity and Specificity


  • Dietary Fats
  • Calcium-Transporting ATPases