A cell cycle-regulated GATA factor promotes centromeric localization of CENP-A in fission yeast

Mol Cell. 2003 Jan;11(1):175-87. doi: 10.1016/s1097-2765(03)00011-x.


CENP-A, the centromere-specific histone H3 variant, plays a crucial role in organizing kinetochore chromatin for precise chromosome segregation. We have isolated Ams2, a Daxx-like motif-containing GATA factor, and histone H4, as multicopy suppressors of cnp1-1, an S. pombe CENP-A mutant. While depletion of Ams2 results in the reduction of CENP-A binding to the centromere and chromosome missegregation, increasing its dosage restores association of a CENP-A mutant protein with centromeres. Conversely, overexpression of CENP-A or histone H4 suppresses an ams2 disruptant. The intracellular amount of Ams2 thus affects centromeric nucleosomal constituents. Ams2 is abundant in S phase and associates with chromatin, including the central centromeres through binding to GATA-core sequences. Ams2 is thus a cell cycle-regulated GATA factor that is required for centromere function.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Motifs
  • Amino Acid Sequence
  • Animals
  • Cell Cycle / physiology*
  • Centromere / metabolism*
  • Chromosomal Proteins, Non-Histone / metabolism*
  • Chromosomes / genetics
  • Chromosomes / metabolism
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • GATA Transcription Factors
  • Histones / metabolism
  • Humans
  • In Situ Hybridization, Fluorescence
  • Molecular Sequence Data
  • Phenotype
  • Protein Binding
  • Schizosaccharomyces / cytology
  • Schizosaccharomyces / physiology*
  • Schizosaccharomyces pombe Proteins / chemistry
  • Schizosaccharomyces pombe Proteins / genetics
  • Schizosaccharomyces pombe Proteins / metabolism*
  • Sequence Alignment


  • Ams2 protein, S pombe
  • Chromosomal Proteins, Non-Histone
  • Cnp1 protein, S pombe
  • DNA-Binding Proteins
  • GATA Transcription Factors
  • Histones
  • Schizosaccharomyces pombe Proteins