Cloning and identification of a novel variant of human vascular endothelial growth factor

J Tongji Med Univ. 2001;21(3):191-4. doi: 10.1007/BF02886426.

Abstract

A novel variant of human vascular endothelial growth factor (h'VEGF165) cDNA was amplified by nested PCR method from the HL601 cells and was cloned into a eukaryotic expressing vector pcDNA3 to construct a recombinant plasmid pCD-h'VEGF165. The amplified h'VEGF165 cDNA fragment was identified by enzyme digestion and DNA sequencing methods. Also, wild-type hVEGF165 cDNA was obtained, identified and cloned into a eukaryotic expressing vector pcDNA3 by using the same methods. The results of DNA sequencing showed that h'VEGF165 cDNA cloned from HL601 was 600 bp in size with 8% of the base sequence in h'VEGF165 cDNA being changed as compared with the base sequence in the wild-type hVEGF165 cDNA. The results of sequencing of hVEGF165 which was cloned from HL60 by us were consistent with the reports completely.

MeSH terms

  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Genetic Variation*
  • HL-60 Cells / metabolism
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • RNA, Messenger / analysis
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Vascular Endothelial Growth Factor A / analysis
  • Vascular Endothelial Growth Factor A / biosynthesis
  • Vascular Endothelial Growth Factor A / genetics*

Substances

  • DNA, Complementary
  • RNA, Messenger
  • Vascular Endothelial Growth Factor A