Antihyperglycaemic effect of Cassia auriculata in experimental diabetes and its effects on key metabolic enzymes involved in carbohydrate metabolism

Clin Exp Pharmacol Physiol. Jan-Feb 2003;30(1-2):38-43. doi: 10.1046/j.1440-1681.2003.03785.x.

Abstract

1. In experimental diabetes, enzymes of glucose and fatty acid metabolism are markedly altered. Persistent hyperglycaemia is a major contributor to such metabolic alterations, which lead to the pathogenesis of diabetic complications. To our knowledge, there are no available reports on the enzymes of hepatic glucose metabolism of Cassia auriculata flower against diabetes. The present study was designed to study the effect of Cassia auriculata flower extract (CFEt) on hepatic glycolytic and gluconeogenic enzymes. 2. Streptozotocin diabetic rats were given CFEt (0.15, 0.30 and 0.45 g/kg) or 600 microg/kg glibenclamide for 30 days. At the end of 30 days, blood glucose, plasma insulin, haemoglobin, glycosylated haemoglobin, glycolytic and gluconeogenic enzymes were assessed. 3. Administration of CFEt at 0.45 g/kg significantly decreased blood glucose, glycosylated haemoglobin and gluconeogenic enzymes and increased plasma insulin, haemoglobin and hexokinase activity. Similarly, administration of glibenclamide showed a significant effect; however, CFEt at 0.15 and 0.30 g/kg did not show any significant effect. 4. In conclusion, the observations show that the aqueous extract of CFEt possesses an antihyperglycaemic effect and suggest that enhanced gluconeogenesis during diabetes is shifted towards normal and that the extract enhances the utilization of glucose through increased glycolysis. The effect of CFEt was more prominent than that of glibenclamide.

MeSH terms

  • Animals
  • Blood Glucose / metabolism
  • Cassia / chemistry*
  • Diabetes Mellitus, Experimental / complications
  • Diabetes Mellitus, Experimental / drug therapy*
  • Diabetes Mellitus, Experimental / enzymology
  • Diabetes Mellitus, Experimental / metabolism*
  • Dose-Response Relationship, Drug
  • Flowers / chemistry
  • Gluconeogenesis / drug effects
  • Glucose Tolerance Test
  • Glyburide / pharmacology
  • Glycated Hemoglobin A / metabolism
  • Glycolysis* / drug effects
  • Hemoglobins / metabolism
  • Hyperglycemia / complications
  • Hyperglycemia / drug therapy*
  • Hyperglycemia / enzymology
  • Hyperglycemia / metabolism
  • Hypoglycemic Agents / pharmacology
  • Insulin / blood
  • Male
  • Phytotherapy
  • Plant Extracts / chemistry
  • Plant Extracts / therapeutic use*
  • Rats
  • Rats, Wistar

Substances

  • Blood Glucose
  • Glycated Hemoglobin A
  • Hemoglobins
  • Hypoglycemic Agents
  • Insulin
  • Plant Extracts
  • Glyburide