[Analysis of soybean lecithin by supercritical fluid chromatography]

Abstract

Separation of six phospholipids, phosphatidyl choline (PC), phosphatidyl-ethanolamine (PE), phosphatidyl-serine(PS), phosphatidyl-inositol(PI), phosphotidic acid(PA), lyso-phosphatidyl-choline(lyso-PC), in soybean lecithin with supercritical fluid chromatography was achieved within 15 min. C18 column was used and carbon dioxide modified by ethanol containing 0.05% (V/V) triethylamine was chosen as the mobile phase. Effects of the composition of mobile phase, temperature and pressure were studied. The quantitative analysis of PC has been achieved with external standard method. The calibration curve for PC was linear in the range between 0.020 g/L-0.075 g/L and the detection limit was 0.2 microgram. This method has been applied to the analysis of PC in soybean lecithin.