Herpes simplex virus type II is not a cofactor to human papillomavirus in cancer of the uterine cervix

Am J Obstet Gynecol. 2003 Jan;188(1):129-34. doi: 10.1067/mob.2003.66.

Abstract

Objective: Cells that were cotransfected with herpes simplex virus-16 and the herpes simplex virus type 2 Xho -2 DNA induce tumors in nude mice. In a cross-sectional study, we investigated the role of herpes simplex virus type 2 as a cofactor to human papillomavirus in cervical cancer.

Study design: Cervical cells that were obtained with an endocervical Cytobrush brush (Medscand) from 439 women (50 women with cancer lesions, 65 women with high-grade squamous intraepithelial lesions, 80 women with low-grade squamous intraepithelial lesions, 244 healthy subjects) and DNA that was extracted from 150 cervical cancer biopsy specimens were analyzed with polymerase chain reaction for herpes simplex virus type 2 Xho -2 and Bgl IIC transforming DNA sequences.

Results: All 439 cervical samples and 150 cervical cancer biopsy specimens tested negative for herpes simplex virus type 2 Xho -2 and Bgl IIC DNA by polymerase chain reaction. Overall, none of 200 samples (0%) from women with invasive cervical cancer contained herpes simplex virus type 2 Xho -2 or Bgl IIC DNA (95% CI, 0.0-1.8).

Conclusion: Although herpes simplex virus type 2 Bgl IIN transforms epithelial cells in vitro, it was not detected in cervical cancer specimens.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / virology
  • Adolescent
  • Adult
  • Aged
  • Carcinoma, Squamous Cell
  • Cervical Intraepithelial Neoplasia / virology
  • Chlamydia trachomatis / genetics
  • DNA, Bacterial / analysis
  • DNA, Viral / isolation & purification
  • Deoxyribonucleases, Type II Site-Specific
  • Female
  • Herpesvirus 2, Human / genetics
  • Herpesvirus 2, Human / isolation & purification*
  • Humans
  • Middle Aged
  • Papillomaviridae / isolation & purification*
  • Polymerase Chain Reaction
  • Uterine Cervical Neoplasms / virology*

Substances

  • DNA, Bacterial
  • DNA, Viral
  • endodeoxyribonuclease BstYI
  • Deoxyribonucleases, Type II Site-Specific