The neuronal targets for GABAergic reticulospinal inhibition that stops swimming in hatchling frog tadpoles

J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2003 Jan;189(1):29-37. doi: 10.1007/s00359-002-0372-0. Epub 2002 Nov 30.

Abstract

In most animals locomotion can be started and stopped by specific sensory cues. We are using a simple vertebrate, the hatchling Xenopus tadpole, to study a neuronal pathway that turns off locomotion. In the tadpole, swimming stops when the head contacts solid objects or the water's surface meniscus. The primary sensory neurons are in the trigeminal ganglion and directly excite inhibitory reticulospinal neurons in the hindbrain. These project axons into the spinal cord and release GABA to inhibit spinal neurons and stop swimming. We ask whether there is specificity in the types of spinal neuron inhibited. We used single-neuron recording to determine which classes of spinal neurons receive inhibition when the head skin is pressed. Ventral motoneurons and premotor interneurons involved in generating the swimming rhythm receive reliable GABAergic inhibition. More dorsal inhibitory premotor interneurons are inhibited less reliably and some are excited. Dorsal sensory pathway interneurons that start swimming following a touch to the trunk skin do not appear to receive such inhibition. There is therefore specificity in the formation of descending inhibitory connections so that more ventral neurons producing swimming are most strongly inhibited.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bicuculline / pharmacology
  • Electric Stimulation
  • Excitatory Postsynaptic Potentials / drug effects
  • Excitatory Postsynaptic Potentials / physiology
  • GABA Antagonists / pharmacology
  • Glycine Agents / pharmacology
  • Interneurons / drug effects
  • Larva / drug effects*
  • Membrane Potentials / physiology
  • Motor Neurons / drug effects
  • Neural Inhibition / physiology*
  • Neural Pathways
  • Physical Stimulation
  • Spinal Cord / drug effects*
  • Strychnine / pharmacology
  • Swimming / physiology*
  • Xenopus laevis

Substances

  • GABA Antagonists
  • Glycine Agents
  • Strychnine
  • Bicuculline