E-box is one of potential cis-regulatory elements for the p53 gene. It was previously reported that USF bound to the E-box of the p53 gene. Recently, we demonstrated that an 80-kDa protein other than USF bound to the E-box and activated the transcription of the p53 gene. In the present study, the 80-kDa protein was partially purified and characterized. First, we confirmed that nuclear factors bound to the E-box in sequence-specific manner by the oligonucleotide competition assay. The binding protein to the E-box was partially purified by a sequence-specific DNA affinity chromatography. The active fraction was analyzed by SDS-PAGE and southwestern blotting assay, which showed that the 80-kDa protein was enriched. The binding activity of the 80-kDa protein was not decreased in the presence of 1.4 M urea. In addition, the binding activity was stable up to 50 degrees C. Treatment of EDTA showed that the 80-kDa protein did not require divalent cation such as Mg2+ for the maximum DNA binding activity. The competition assay with non-specific competitor, poly (dI-dC) showed that the 80-kDa protein had high affinity to its binding site. These biochemical properties provide useful insights into the 80-kDa nuclear factor binding to the p53 promoter.