Lemon flavonoid, eriocitrin, suppresses exercise-induced oxidative damage in rat liver

Life Sci. 2003 Feb 21;72(14):1609-16. doi: 10.1016/s0024-3205(02)02443-8.


To examine the preventive effect of the lemon flavonoid, eriocitrin (eriodictyol 7-O-rutinoside), on oxidative stress during acute exercise in vivo, levels of N( epsilon )- (hexanoyl)lysine, HEL; o,o-dityrosine, DT; and nitrotyrosine, NT, as oxidative stress markers, were determined by ELISA in livers of trained rats in addition to thiobarbituric acid-reactive substance (TBARS). Eriocitrin administration prior to exercise significantly suppressed the increases in TBARS caused by lipid peroxidation during acute exercise. The contents of HEL, DT, and NT in rat liver increased dramatically by exercise without eriocitrin administration. However, these increases were significantly suppressed by eriocitrin administration before exercise. Moreover, in this study, to clarify whether eriocitrin influences glutathione metabolite system that is considered to be important for a defense against the damage by oxidative stress, the levels of glutathione in rat liver were determined during exercise. The level of reduced glutathione after exercise was maintained by administration of eriocitrin. The increase in the concentration of oxidized glutathione caused by exercise was significantly suppressed by eriocitrin. This result suggested that eriocitrin might play an important role in the control of the change in glutathione redox status in rat liver during exercise. These findings showed that eriocitrin was effective in the prevention of oxidative damages caused by acute exercise-induced oxidative stress.

MeSH terms

  • Animals
  • Enzyme-Linked Immunosorbent Assay
  • Flavanones*
  • Glutathione / metabolism*
  • Hesperidin / analogs & derivatives*
  • Hesperidin / pharmacology*
  • Hexanols / metabolism
  • Lipid Peroxidation / drug effects*
  • Liver / drug effects*
  • Liver / metabolism
  • Lysine / chemistry
  • Male
  • Oxidative Stress / drug effects*
  • Physical Conditioning, Animal*
  • Rats
  • Rats, Wistar
  • Thiobarbituric Acid Reactive Substances / metabolism
  • Tyrosine / analogs & derivatives*
  • Tyrosine / metabolism


  • Flavanones
  • Hexanols
  • Thiobarbituric Acid Reactive Substances
  • 3-nitrotyrosine
  • Tyrosine
  • eriocitrin
  • dityrosine
  • Hesperidin
  • Glutathione
  • Lysine