Interaction between dental metals and antioxidants, assessed by cytotoxicity assay and ESR spectroscopy

Anticancer Res. Nov-Dec 2002;22(6C):4017-22.

Abstract

Among dental metals, copper showed the highest cytotoxicity against human oral squamous cell carcinoma and human submandibular gland carcinoma cells, followed by palladium-alloy, gold and silver. Normal human cells (gingival fibroblast, pulp cells, periodontal ligament fibroblast) were relatively resistant to these metals. The palladium-alloy failed to induce internucleosomal DNA fragmentation, a biochemical hallmark of apoptosis, in human promyelocytic leukemic HL-60 cells. The cytotoxic activity of the palladium-alloy was significantly reduced by a non-cytotoxic concentration of N-acetyl-L-cysteine, or more efficiently by sodium ascorbate. However, higher concentrations of sodium ascorbate enhanced the cytotoxic activity of palladium-alloy. ESR spectroscopy showed that the palladium-alloy enhanced the intensity of ascorbate radical, suggesting the possible interaction between metals and antioxidants. All metals, except copper, did not significantly affect the generation of superoxide anion (by hypoxanthine-xanthine oxidase reaction), hydroxyl radical (by Fenton reaction) and nitric oxide (from NOC-7 in the presence of C-PTIO). These data demonstrate for the first time that antioxidants modify the biological activity of dental metals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / pharmacology*
  • Ascorbic Acid / pharmacology
  • Carcinoma, Squamous Cell / drug therapy
  • Copper / chemistry
  • Copper / metabolism
  • Copper / pharmacology
  • Dental Materials / chemistry
  • Dental Materials / metabolism
  • Dental Materials / pharmacology*
  • Drug Interactions
  • Electron Spin Resonance Spectroscopy
  • Fibroblasts / drug effects
  • Free Radicals / chemistry
  • Free Radicals / metabolism
  • Gingiva / cytology
  • Gingiva / drug effects
  • Gold / chemistry
  • Gold / metabolism
  • Gold / pharmacology
  • HL-60 Cells / drug effects
  • Humans
  • Hypoxanthine / chemistry
  • Hypoxanthine / metabolism
  • Metals / chemistry
  • Metals / metabolism
  • Metals / pharmacology*
  • Mouth Neoplasms / drug therapy
  • Palladium / chemistry
  • Palladium / metabolism
  • Palladium / pharmacology
  • Periodontal Ligament / cytology
  • Periodontal Ligament / drug effects
  • Reactive Oxygen Species / chemistry
  • Reactive Oxygen Species / metabolism
  • Silver / chemistry
  • Silver / metabolism
  • Silver / pharmacology
  • Submandibular Gland Neoplasms / drug therapy
  • Tumor Cells, Cultured
  • Xanthine Oxidase / chemistry
  • Xanthine Oxidase / metabolism

Substances

  • Antioxidants
  • Dental Materials
  • Free Radicals
  • Metals
  • Reactive Oxygen Species
  • Hypoxanthine
  • Silver
  • Palladium
  • Gold
  • Copper
  • Xanthine Oxidase
  • Ascorbic Acid