Expression, crystallization and activities of the two family 11 aldo-keto reductases from Bacillus subtilis

Acta Crystallogr D Biol Crystallogr. 2003 Feb;59(Pt 2):375-7. doi: 10.1107/s0907444902072001. Epub 2003 Jan 23.

Abstract

Two members of the aldo-keto reductase family 11 from Bacillus subtilis have been crystallized and their oxidoreductase activity confirmed. AKR11A is a protein induced by inositol and repressed by glucose. AKR11B is induced when the cell is stressed by heat, acid, ethanol, starvation or osmotic shock and is therefore classified as a general stress protein. The expected NADPH-dependent sugar reductase activities for both proteins have been confirmed kinetically with several substrates. AKR11B exhibited typical aldo-keto reductase kinetics. However, only trace activity was found in AKR11A. To examine the effects of differences in sequence on the structures and functions of these enzymes, a crystallographic study has been initiated. AKR11A has been crystallized in its apo form and AKR11B crystals were obtained in complex with NADP(+).

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alcohol Oxidoreductases / chemistry*
  • Alcohol Oxidoreductases / genetics
  • Alcohol Oxidoreductases / metabolism*
  • Bacillus subtilis / enzymology*
  • Crystallization
  • Crystallography, X-Ray
  • Glucose / pharmacology
  • Glyceraldehyde / metabolism
  • Inositol / pharmacology
  • Kinetics
  • NADP / chemistry
  • NADP / metabolism

Substances

  • Glyceraldehyde
  • Inositol
  • NADP
  • Alcohol Oxidoreductases
  • Glucose