Five new strains of Bacillus thuringiensis(Bt) from soil in China were classified. 5 new H-serotypes: H42, H43, H56, H60 and H62 were identified. There were some distinction in the morphology, biochemical reaction and toxicity against species of insect. Five new Bt subspecies were identified as follows: Strain YGd22-03 Bacillus thuringiensis subsp. jinghongiensis, H42; Strain KK31-01 Bacillus thuringiensis subsp. guiyangiensis, H43; Strain SCg04-02 B. thuringiensis subsp. rongseni, H56; Strain NXP15-04 B. thuringiensis subsp. pingluonsis, H60; Strain HZ39-04 B. thuringiensis subsp. zhaodongensis, H62.