Spatial variation of plasma flow in the oxazolone-stimulated microcirculation

Inflamm Res. 2002 Dec;51(12):572-8. doi: 10.1007/pl00012431.


Introduction: In cutaneous lymphocytic inflammation, enhanced regional blood flow is suggested by persistent erythema and warmth. Direct assessment of the microcirculation, however, has been limited by tissue edema and skin thickness.

Methods: To assess the microcirculatory adaptations to the epicutaneous antigen oxazolone, we studied the first pass kinetics and microvascular topography of the inflammatory skin microcirculation using a specially adapted epi-illumination intravital microscopy system. The fluorescence intravital videomicroscopy and streaming image acquisition of fluorescein-labeled dextran (approximately 500,000 MW) injections were used to assess changes in plasma flow.

Results: Direct plasma tracer injections of both the oxazolone-stimulated and control microcirculation demonstrated comparable transit times (leading edge and intensity-weighted peak times) from the carotid artery to the superficial vascular plexus (p > 0.05). In contrast to transit times, continuous infusion of the plasma tracer demonstrated a significant increase in the delivery of the fluorescein-labeled dextran to the oxazolone-stimulated microcirculation. Quantitative morphometry of intravital microscopic images demonstrated a 2.2-fold increase in the mean diameter of vessels in the superficial vascular plexus (p < 0.01). Further, fluorescence intensity mapping indicated that the increase was associated with increased perfusion of focal regions of the superficial vascular plexus (p < 0.001).

Conclusions: These results indicate that the oxazolone-stimulated adaptations of the inflammatory microcirculation include both microvascular dilatation and the redistribution of plasma flow.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Capillaries / anatomy & histology
  • Capillaries / drug effects
  • Coloring Agents
  • Eosine Yellowish-(YS)
  • Erythema / pathology
  • Fluorescent Dyes
  • Hematoxylin
  • Lymphocytes / drug effects
  • Microcirculation / drug effects
  • Microscopy, Fluorescence
  • Oxazoles / pharmacology*
  • Regional Blood Flow / drug effects
  • Sheep
  • Skin / blood supply*
  • Skin / drug effects
  • Stimulation, Chemical


  • Coloring Agents
  • Fluorescent Dyes
  • Oxazoles
  • Eosine Yellowish-(YS)
  • Hematoxylin