U2 small nuclear ribonucleoprotein particle (snRNP) auxiliary factor of 65 kDa, U2AF65, can promote U1 snRNP recruitment to 5' splice sites

Biochem J. 2003 May 15;372(Pt 1):235-40. doi: 10.1042/BJ20021202.

Abstract

The splicing factor U2AF(65), U2 small nuclear ribonucleoprotein particle (snRNP) auxillary factor of 65 kDa, binds to pyrimidine-rich sequences at 3' splice sites to recruit U2 snRNP to pre-mRNAs. We report that U2AF(65) can also promote the recruitment of U1 snRNP to weak 5' splice sites that are followed by uridine-rich sequences. The arginine- and serine-rich domain of U2AF(65) is critical for U1 recruitment, and we discuss the role of its RNA-RNA annealing activity in this novel function of U2AF(65).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Nucleus / metabolism
  • HeLa Cells
  • Humans
  • Nuclear Proteins*
  • Protein Binding
  • Protein Structure, Tertiary
  • RNA / metabolism
  • RNA Splice Sites / physiology*
  • Ribonucleoprotein, U1 Small Nuclear / metabolism*
  • Ribonucleoproteins / metabolism*
  • Splicing Factor U2AF

Substances

  • Nuclear Proteins
  • RNA Splice Sites
  • Ribonucleoprotein, U1 Small Nuclear
  • Ribonucleoproteins
  • Splicing Factor U2AF
  • U2AF2 protein, human
  • RNA