Effect of aging and late onset dietary restriction on antioxidant enzymes and proteasome activities, and protein carbonylation of rat skeletal muscle and tendon

Exp Gerontol. 2002 Dec;37(12):1423-30. doi: 10.1016/s0531-5565(02)00116-x.


Many studies have shown that lifelong dietary restriction (DR) can retard aging processes. Very few reports, however, are found that examined the effect of late onset DR on biochemical parameters in aging animals [Goto, S., Takahashi, R., Araki, S., Nakamoto, H., 2002b. Dietary restriction initiated in late adulthood can reverse age-related alterations of protein and protein metabolism. Ann. NY Acad. Sci. 959, 50-56]. We studied the effect of every-other-day feeding, initiated at the age of 26.5 months and continued for 3.5 months, on antioxidant enzymes, protein carbonyls, and proteasomes of the gastrocnemius muscle and tendon in rats. Age-related increase in the activity and content of Cu, Zn-SOD and the content of Mn-SOD was attenuated by the DR in both tissues. The same was true for glutathione peroxidase and catalase activities. Significant increase with age in protein reactive carbonyl derivatives (RCD) in the tendon was noted that was partially reversed by the DR. No significant change of RCD, however, was observed in the skeletal muscle. The age-related and DR-induced changes of the RCD in the tendon appeared to be associated with proteasome activity that decreases with age and increases by the DR. It is suggested that the late onset DR can have beneficial effects on the locomotive functions by reducing age-associated potentially detrimental oxidative protein damage in the tendon.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Achilles Tendon / metabolism*
  • Aging / metabolism*
  • Animals
  • Antioxidants / metabolism
  • Catalase / metabolism
  • Cysteine Endopeptidases / metabolism*
  • Food Deprivation / physiology*
  • Glutathione Peroxidase / metabolism
  • Male
  • Multienzyme Complexes / metabolism*
  • Muscle, Skeletal / metabolism*
  • Oxidation-Reduction
  • Oxidative Stress / physiology
  • Proteasome Endopeptidase Complex
  • Rats
  • Rats, Inbred F344
  • Superoxide Dismutase / metabolism


  • Antioxidants
  • Multienzyme Complexes
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Cysteine Endopeptidases
  • Proteasome Endopeptidase Complex