Reduction of beta-amyloid plaques in brain of transgenic mouse model of Alzheimer's disease by EFRH-phage immunization

Vaccine. 2003 Mar 7;21(11-12):1060-5. doi: 10.1016/s0264-410x(02)00609-6.

Abstract

Antibodies to the epitope EFRH, representing residues 3-6 within the beta-amyloid (Abeta) sequence, were previously shown to affect the solubility and disaggregation of Abeta fibrils in vitro. Here, we describe a novel method of immunization, using as antigen the EFRH peptide displayed on the surface of the filamentous phage. The EFRH phage evoked effective auto-immune antibodies in amyloid precursor protein [V717I] (APP[V717I]) transgenic mice that recapitulate the amyloid plaques and vascular pathology of Alzheimer's disease (AD). The immunization provoked a considerable reduction in the number of Abeta amyloid plaques in the brain of the transgenic mice and may serve as the basis for anti-Abeta vaccine.

Publication types

  • Evaluation Study

MeSH terms

  • Alzheimer Disease / drug therapy
  • Alzheimer Disease / pathology
  • Alzheimer Disease / prevention & control*
  • Alzheimer Vaccines / immunology*
  • Amyloid beta-Peptides / analysis*
  • Amyloid beta-Peptides / genetics
  • Amyloid beta-Peptides / immunology
  • Animals
  • Autoantibodies / biosynthesis
  • Autoantibodies / immunology
  • Brain Chemistry*
  • Epitopes / genetics
  • Epitopes / immunology*
  • Humans
  • Immunization
  • Inovirus
  • Mice
  • Mice, Transgenic
  • Microscopy, Electron
  • Models, Animal
  • Peptide Library
  • Plaque, Amyloid / chemistry*
  • Plaque, Amyloid / pathology
  • Vaccines, Synthetic / therapeutic use

Substances

  • Alzheimer Vaccines
  • Amyloid beta-Peptides
  • Autoantibodies
  • Epitopes
  • Peptide Library
  • Vaccines, Synthetic