cDNA Cloning and Characterization of the Third Isoform of Human Peroxin Pex11p

Biochem Biophys Res Commun. 2003 Jan 24;300(4):819-23. doi: 10.1016/s0006-291x(02)02936-4.

Abstract

We have cloned a human cDNA encoding an isoform of the peroxin Pex11p, termed Pex11pgamma, by BLASTP homology search on eukaryotic protein database and reverse transcription (RT)-PCR of human fibroblast RNA. Pex11pgamma was 241 amino-acid long, with two putative transmembrane segments, showing 22% and 23% amino-acid identity to Pex11palpha and Pex11pbeta, respectively. PEX11gamma gene was located on chromosome 19, distinct from PEX11alpha and PEX11beta. Pex11pgamma was found to be a peroxisomal membrane protein, as assessed by colocalization with peroxisome targeting signal type 1 (PTS1)-proteins, in epitope-tagged Pex11pgamma-expressing Chinese hamster ovary cells. Pex11pgamma exposes both of the N- and C-terminal parts to the cytosol. PEX11gamma was not induced in rats by treatment of clofibrate, a peroxisome proliferator, similar to constitutively expressed PEX11beta but in contrast to inducible PEX11alpha.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • CHO Cells
  • Clofibrate / pharmacology
  • Cloning, Molecular
  • Cricetinae
  • DNA, Complementary
  • Humans
  • Hypolipidemic Agents / pharmacology
  • Liver / drug effects
  • Liver / physiology
  • Membrane Proteins / chemistry
  • Membrane Proteins / classification
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Molecular Sequence Data
  • Peroxins
  • Protein Isoforms / chemistry
  • Protein Isoforms / classification
  • Protein Isoforms / genetics
  • Protein Isoforms / metabolism
  • Rats
  • Sequence Alignment

Substances

  • DNA, Complementary
  • Hypolipidemic Agents
  • Membrane Proteins
  • PEX11A protein, human
  • PEX11B protein, human
  • Peroxins
  • Pex11a protein, rat
  • Protein Isoforms
  • Clofibrate