Structural Characterization of the O-chain Polysaccharide Isolated From Bordetella Avium ATCC 5086: Variation on a theme(1)

FEBS Lett. 2003 Jan 30;535(1-3):11-6. doi: 10.1016/s0014-5793(02)03843-7.

Abstract

The O-chain polysaccharide (O-PS) of Bordetella avium was isolated from the lipopolysaccharide by mild acid hydrolysis to remove the lipid A, followed by hydrofluorolysis to remove the lipopolysaccharide core oligosaccharide leaving a residual O-PS for structural analysis. High resolution (1)H and (13)C NMR and MALDI studies showed the O-chain to be a polymer composed of 1,4-linked 2-acetamidino-3-[3-hydroxybutanamido]-2,3-dideoxy-beta-D-glucopyranosyluronic acid residues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3-Hydroxybutyric Acid / chemistry
  • Acetamides / chemistry
  • Bordetella / chemistry*
  • Carbon Isotopes
  • Electrophoresis, Polyacrylamide Gel
  • Hydrofluoric Acid / chemistry
  • Hydrolysis
  • Lipopolysaccharides / chemistry
  • Magnetic Resonance Spectroscopy
  • Molecular Structure
  • Nuclear Magnetic Resonance, Biomolecular
  • Polysaccharides, Bacterial / chemistry*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

  • Acetamides
  • Carbon Isotopes
  • Lipopolysaccharides
  • Polysaccharides, Bacterial
  • acetamide
  • Hydrofluoric Acid
  • 3-Hydroxybutyric Acid