Molecular and serological characterization of sporadic acute hepatitis E in a Japanese patient infected with a genotype III hepatitis E virus in 1993

J Gen Virol. 2003 Feb;84(Pt 2):421-427. doi: 10.1099/vir.0.18802-0.


Serum samples collected periodically from a 40-year-old Japanese woman who had not travelled abroad and who had contracted sporadic acute hepatitis E in 1993 were semi-quantitatively tested by enzyme immunoassay for IgM, IgA and IgG antibodies to hepatitis E virus (HEV). Anti-HEV IgM and IgA antibody levels were the highest (1 : 2400 dilution and 1 : 3400 dilution, respectively) on day 9 after the onset of hepatitis and then decreased rapidly in a parallel manner. Anti-HEV IgG antibody levels were the highest (1 : 17000 dilution) on day 145 and then decreased gradually but remained at high titres (1 : 2200 dilution) even 8.7 years after the onset of hepatitis. An HEV isolate, HE-JA10, recovered from the patient's serum at admission was closely related to a genotype III strain isolated in the United States (US1), with 92.2% identity over the full-length genome, and was most closely related to the JMY-Haw isolate of Japanese origin (95.4% identity).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Base Sequence
  • Female
  • Genotype
  • Hepatitis Antibodies / blood*
  • Hepatitis E / virology
  • Hepatitis E virus / classification*
  • Hepatitis E virus / genetics
  • Hepatitis E virus / immunology*
  • Humans
  • Immunoglobulin A / blood
  • Immunoglobulin G / blood
  • Immunoglobulin M / blood
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Viral / blood
  • Sequence Analysis, DNA


  • Hepatitis Antibodies
  • Immunoglobulin A
  • Immunoglobulin G
  • Immunoglobulin M
  • RNA, Viral

Associated data

  • GENBANK/AB089824