Fission yeast synaptobrevin is involved in cytokinesis and cell elongation

Biochem Biophys Res Commun. 2003 Feb 14;301(3):641-5. doi: 10.1016/s0006-291x(03)00017-2.

Abstract

Synaptobrevin is a vesicle-associated membrane protein playing an essential role in regulated vesicle transport. In this study, we characterized Syb1, synaptobrevin of Schizosaccharomyces pombe. Syb1 was located on various sizes of vesicle-like structures in the cytoplasm and enriched in the medial region and cell ends. Transport of Syb1 to the medial region was mainly dependent on F-actin and Myo52/Myo4. Syb1 is essential for cell viability and most of the syb1-null cells showed a round or short cylindrical form. These results suggest that Syb1 is involved in membrane trafficking of cytokinesis and cell elongation.

MeSH terms

  • Amino Acid Sequence
  • Cell Division
  • Cell Size
  • Cytoplasmic Vesicles / chemistry
  • Membrane Proteins / analysis
  • Membrane Proteins / chemistry
  • Membrane Proteins / physiology*
  • Molecular Sequence Data
  • Protein Structure, Tertiary
  • R-SNARE Proteins
  • Schizosaccharomyces / chemistry
  • Schizosaccharomyces / cytology*
  • Schizosaccharomyces pombe Proteins / analysis
  • Schizosaccharomyces pombe Proteins / chemistry
  • Schizosaccharomyces pombe Proteins / physiology*
  • Sequence Homology, Amino Acid

Substances

  • Membrane Proteins
  • R-SNARE Proteins
  • Schizosaccharomyces pombe Proteins
  • Syb1 protein, S pombe