A sense of closeness: protein detection by proximity ligation

Curr Opin Biotechnol. 2003 Feb;14(1):82-6. doi: 10.1016/s0958-1669(02)00011-3.

Abstract

Highly specific and sensitive procedures will be required to evaluate proteomes. Proximity ligation is a recently introduced mechanism for protein analysis. In this technique, the convergence of sets of protein-binding reagents on individual target molecules juxtaposes attached nucleic acid sequences. Through a ligation reaction a DNA reporter sequence is created, which can be amplified. The procedure thus encodes detected proteins as specific nucleic acid sequences in what may be viewed as a reverse translation reaction.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Base Sequence
  • DNA Probes*
  • Enzyme-Linked Immunosorbent Assay / methods
  • Macromolecular Substances
  • Molecular Probe Techniques*
  • Nucleic Acid Amplification Techniques / methods
  • Oligonucleotides / chemistry
  • Protein Binding
  • Proteins / analysis
  • Proteins / chemistry*
  • Proteins / genetics
  • Sequence Analysis, Protein / methods*

Substances

  • DNA Probes
  • Macromolecular Substances
  • Oligonucleotides
  • Proteins