Trypan blue not toxic for retinal pigment epithelium in vitro

Am J Ophthalmol. 2003 Feb;135(2):234-6. doi: 10.1016/s0002-9394(02)01891-3.


Purpose: To investigate whether trypan blue has a toxic effect on cultured retinal pigment epithelial (retinal pigment epithelium) cells.

Design: Experimental study with a direct live/dead cell staining technique using fluorescent dyes.

Methods: Cultured human retinal pigment epithelium cells were exposed for 5 minutes to various concentrations of trypan blue (0.06%, 0.15%, 0.30%), and cell viability was confocally measured.

Results: No increased cell death was found in cultures incubated in any of the trypan blue concentrations used.

Conclusion: These findings indicate that a short exposure of trypan blue does not have a toxic effect on cultured retinal pigment epithelium cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Survival / drug effects
  • Cells, Cultured
  • Coloring Agents / pharmacology*
  • Humans
  • Microscopy, Confocal
  • Pigment Epithelium of Eye / cytology
  • Pigment Epithelium of Eye / drug effects*
  • Trypan Blue / pharmacology*


  • Coloring Agents
  • Trypan Blue