Acteoside from Cistanche salsa inhibits apoptosis by 1-methyl-4-phenylpyridinium ion in cerebellar granule neurons

Planta Med. 2003 Jan;69(1):65-6. doi: 10.1055/s-2003-37029.

Abstract

In this study we assessed the effect of acteoside that significantly improved cell viability and inhibited lactate dehydrogenase (LDH) release. Furthermore acteoside prevented a neurotoxin 1-methyl-4-phenylpyridinium ion (MPP+)-induced apoptosis in CGNs. Accordingly, our flow cytometric analysis of CGNs after acteoside treatment revealed a decrease in the number of the MPP+-induced apoptotic cells (P < 0.001). Western blot analysis demonstrated that acteoside inhibits the active caspase-3 fragment (17 kDa) (P < 0.001) and the proteolytic poly (ADP-ribose) polymerase (PARP) fragment (85 kDa) expression (P < 0.001) following MPP + treatment in CGNs. We conclude that acteoside prevents the MPP+-induced apoptosis and inhibits the apoptosis-related pathway.

Publication types

  • Letter

MeSH terms

  • 1-Methyl-4-phenylpyridinium / pharmacology
  • Animals
  • Apoptosis / drug effects*
  • Cell Survival / drug effects
  • Cerebellum / cytology
  • China
  • Cistanche / chemistry*
  • Glucosides / chemistry
  • Glucosides / pharmacology*
  • Neurons / drug effects*
  • Phenols / chemistry
  • Phenols / pharmacology*
  • Rats

Substances

  • Glucosides
  • Phenols
  • acteoside
  • 1-Methyl-4-phenylpyridinium