Genomic organization, splice products and mouse chromosomal localization of genes for transcription factor Nuclear Factor One

Gene. 2003 Jan 30;304:171-81. doi: 10.1016/s0378-1119(02)01204-0.


Transcription factor Nuclear Factor One (NFI) proteins are derived from a small family of four vertebrate genes (NFIA, B, C and X), all of which produce a fair number of protein variants by alternative splicing. In order to ultimately locate RNA signal sequences around exon/intron borders for the production of regulated splice variants, we have determined the exon structure of the chicken NFIB gene as the last of the four vertebrate genes for which the gene structure was not yet elucidated. This made it possible to compile nine newly isolated and sequenced mouse NFI cDNA sequences together with all previously available ones and to deduce corresponding splicing patterns for the orthologous vertebrate genes of all four paralogous gene types. Results from the analysis of alternative splicing and of NFI gene mapping in the genome of human and mouse argue for a phylogenetic route in which the four vertebrate NFI genes result from a single duplication of a genomic segment containing two NFI intermediate genes rather than from two independent duplications of two separated single ancestor genes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing*
  • Amino Acid Sequence
  • Animals
  • CCAAT-Enhancer-Binding Proteins / genetics*
  • Chromosome Mapping
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • DNA-Binding Proteins*
  • Exons
  • Female
  • Genes / genetics
  • In Situ Hybridization, Fluorescence
  • Introns
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • NFI Transcription Factors
  • Nuclear Proteins
  • Protein Isoforms / genetics
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Transcription Factors / genetics*
  • Y-Box-Binding Protein 1


  • CCAAT-Enhancer-Binding Proteins
  • DNA, Complementary
  • DNA-Binding Proteins
  • NFI Transcription Factors
  • Nfia protein, mouse
  • Nuclear Proteins
  • Protein Isoforms
  • Transcription Factors
  • Y-Box-Binding Protein 1
  • YBX1 protein, human