The effect of Lopanthus roots extract on the HI humoral immunity and cell-mediated immunity in rat was investigated in this study. The result showed that the Lophanthus roots extract could enhance liver index in rat. By Lophanthus roots extract at the dosage of 2.50 g/kg in rat, the Immunoglobulin G(IgG), immunoglobulin A(IgA) and immunoglobulin M (IgM) were increased, C3 complenment exhanced and have a highly signifcance compared to the cation by the gingseng veinforce pill (P < 0.01). The Lophanthus roots extract could mildly increased effect of rosette formation and lymphocyte transformation, but no statistic sighnificance was found.