Isolation and characterisation of the rabies virus N degrees-P complex produced in insect cells

Virology. 2003 Jan 20;305(2):406-14. doi: 10.1006/viro.2002.1748.

Abstract

When the nucleoprotein (N) of nonsegmented negative-strand RNA viruses is expressed in insect cells, it binds to cellular RNA and forms N-RNA complexes just like viral nucleocapsids. However, in virus-infected cells, N is prevented from binding to cellular RNA because a soluble complex is formed between N and the viral phosphoprotein (P), the N degrees -P complex. N is only released from this complex for binding to newly made viral or complementary RNA. We coexpressed rabies virus N and P proteins in insect cells and purified the N degrees -P complex. Characterisation by gel filtration, polyacrylamide gel electrophoresis, analytical ultracentrifugation, native mass spectroscopy, and electron microscopy showed that the complex consists of one N protein plus two P proteins, i.e., an N degrees -P(2) complex.

MeSH terms

  • Animals
  • Chromatography, Gel
  • Microscopy, Electron
  • Molecular Weight
  • Nucleocapsid / chemistry
  • Nucleocapsid / isolation & purification*
  • Nucleocapsid / physiology
  • Nucleocapsid Proteins
  • Phosphoproteins / chemistry
  • Phosphoproteins / isolation & purification*
  • Phosphoproteins / physiology
  • Recombinant Proteins / isolation & purification
  • Spodoptera
  • Viral Proteins / isolation & purification*

Substances

  • Nucleocapsid Proteins
  • Phosphoproteins
  • Recombinant Proteins
  • Viral Proteins
  • nucleocapsid protein, Rabies virus