RhoD regulates endosome dynamics through Diaphanous-related Formin and Src tyrosine kinase

Nat Cell Biol. 2003 Mar;5(3):195-204. doi: 10.1038/ncb935.


Early endosomes move bidirectionally between the cell periphery and the interior through a mechanism regulated by the low molecular weight GTPase RhoD. Here, we identify a novel splice variant of human Diaphanous, hDia2C, which specifically binds to RhoD and is recruited onto early endosomes. Expression of RhoD and hDia2C induces a striking alignment of early endosomes along actin filaments and reduces their motility. This activity depends on the membrane recruitment and activation of c-Src kinase, thus uncovering a new role in endosome function. Our results define a novel signal transduction pathway, in which hDia2C and c-Src are sequentially activated by RhoD to regulate the motility of early endosomes through interactions with the actin cytoskeleton.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Carrier Proteins / chemistry
  • Carrier Proteins / metabolism
  • Carrier Proteins / physiology*
  • Cloning, Molecular
  • DNA Primers
  • Endocytosis
  • Endosomes / physiology*
  • Formins
  • HeLa Cells
  • Humans
  • Molecular Sequence Data
  • Precipitin Tests
  • Protein Binding
  • Sequence Homology, Amino Acid
  • rho GTP-Binding Proteins / metabolism
  • rho GTP-Binding Proteins / physiology*
  • src-Family Kinases / metabolism
  • src-Family Kinases / physiology*


  • Carrier Proteins
  • DIAPH2 protein, human
  • DNA Primers
  • Formins
  • src-Family Kinases
  • RHOD protein, human
  • rho GTP-Binding Proteins