An Arabidopsis mutant defective in UV-B light-mediated responses

Plant J. 2003 Feb;33(3):591-601. doi: 10.1046/j.1365-313x.2003.01649.x.

Abstract

In plants, low fluences of ultraviolet-B radiation (UV-B; 280-320 nm) trigger the activation of protection mechanisms and various photomorphogenic responses. We established a novel screen to isolate Arabidopsis T-DNA mutants (uli, UV-B light insensitive) with reduced sensitivity towards UV-B light-mediated inhibition of hypocotyl growth. One of the mutants, uli3, shows also a reduced sensitivity of UV-B-induced gene expression events and was, therefore, chosen for further investigation. The ULI3 gene encodes an 80-kDa protein with potential domains for heme- and diacylglycerol-binding. In transiently transfected protoplasts, the ULI3:GFP fusion protein is localised in the cytoplasm but also adjacent to membranes. In etiolated Arabidopsis seedlings irradiated by UV-B, ULI3 mRNA expression is strongly up-regulated. This is caused by elevated transcription as demonstrated using stable transformants where a GUS-reporter was driven by the ULI3-promoter. ULI3 is preferentially expressed in the outer cell layers in leaves, stems and flowers, but not in roots. There is evidence that ULI3 represents a specific component involved in UV-B-mediated signal transduction in higher plants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anthocyanins / metabolism
  • Arabidopsis / genetics*
  • Arabidopsis / metabolism
  • Arabidopsis / radiation effects
  • Arabidopsis Proteins / genetics*
  • Arabidopsis Proteins / metabolism
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Cell Membrane / metabolism
  • Cytoplasm / metabolism
  • Flowers / genetics
  • Flowers / metabolism
  • Gene Expression Regulation, Plant / genetics
  • Gene Expression Regulation, Plant / radiation effects
  • Green Fluorescent Proteins
  • Intracellular Signaling Peptides and Proteins
  • Luminescent Proteins / genetics
  • Luminescent Proteins / metabolism
  • Molecular Sequence Data
  • Mutation*
  • Plant Leaves / genetics
  • Plant Leaves / metabolism
  • Plant Stems / genetics
  • Plant Stems / metabolism
  • Plants, Genetically Modified
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Signal Transduction / genetics
  • Signal Transduction / radiation effects
  • Ultraviolet Rays

Substances

  • Anthocyanins
  • Arabidopsis Proteins
  • Carrier Proteins
  • Intracellular Signaling Peptides and Proteins
  • Luminescent Proteins
  • Recombinant Fusion Proteins
  • ULI3 protein, Arabidopsis
  • Green Fluorescent Proteins

Associated data

  • GENBANK/AF321919