Requirement of autophosphorylated tyrosine 992 of EGF receptor and its docking protein phospholipase C gamma 1 for membrane ruffle formation

FEBS Lett. 2003 Feb 11;536(1-3):71-6. doi: 10.1016/s0014-5793(03)00013-9.


Stimulation of the epidermal growth factor receptor (EGFR) produces membrane ruffles through the small G protein Rac1; however, the signaling pathway from EGFR to Rac1 has not yet been clarified. Here, we show that autophosphorylation of EGFR at tyrosine 992 is required for EGF-induced membrane ruffle formation in CHO cells. Signaling from the autophosphorylated tyrosine 992 appears to be mediated by phospholipase C (PLC) gamma 1. Furthermore, activation of Rac1 by EGF is inhibited by a PLC inhibitor. These results, taken together, suggest that autophosphorylation of EGFR at tyrosine 992 and the subsequent PLC gamma 1 activation transduce the signal to Rac1 to induce membrane ruffle formation.

MeSH terms

  • Animals
  • CHO Cells
  • Cell Membrane / enzymology
  • Cell Membrane / metabolism*
  • Cell Membrane / ultrastructure
  • Cell Surface Extensions / metabolism
  • Cell Surface Extensions / ultrastructure
  • Cricetinae
  • Epidermal Growth Factor / pharmacology
  • ErbB Receptors / chemistry*
  • ErbB Receptors / metabolism*
  • Mitogen-Activated Protein Kinases / metabolism
  • Phospholipase C gamma
  • Phosphorylation
  • Signal Transduction
  • Type C Phospholipases / metabolism*
  • Tyrosine / metabolism*
  • rac1 GTP-Binding Protein / metabolism


  • Tyrosine
  • Epidermal Growth Factor
  • ErbB Receptors
  • Mitogen-Activated Protein Kinases
  • Type C Phospholipases
  • Phospholipase C gamma
  • rac1 GTP-Binding Protein