Cloning and characterization of a gene cluster for geldanamycin production in Streptomyces hygroscopicus NRRL 3602

FEMS Microbiol Lett. 2003 Jan 28;218(2):223-30. doi: 10.1016/S0378-1097(02)01148-5.

Abstract

We illustrate the use of a PCR-based method by which the genomic DNA of a microorganism can be rapidly queried for the presence of type I modular polyketide synthase genes to clone and characterize, by sequence analysis and gene disruption, a major portion of the geldanamycin production gene cluster from Streptomyces hygroscopicus var. geldanus NRRL 3602.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Benzoquinones
  • Cloning, Molecular
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / classification
  • DNA, Bacterial / genetics
  • Gene Expression Regulation, Bacterial
  • Genes, Bacterial*
  • Lactams, Macrocyclic
  • Models, Molecular
  • Molecular Sequence Data
  • Multigene Family*
  • Quinones / isolation & purification
  • Quinones / metabolism*
  • Quinones / pharmacology
  • Streptomyces / genetics*
  • Streptomyces / metabolism

Substances

  • Benzoquinones
  • DNA, Bacterial
  • Lactams, Macrocyclic
  • Quinones
  • geldanamycin