Abstract
We illustrate the use of a PCR-based method by which the genomic DNA of a microorganism can be rapidly queried for the presence of type I modular polyketide synthase genes to clone and characterize, by sequence analysis and gene disruption, a major portion of the geldanamycin production gene cluster from Streptomyces hygroscopicus var. geldanus NRRL 3602.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Benzoquinones
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Cloning, Molecular
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DNA, Bacterial / chemistry
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DNA, Bacterial / classification
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DNA, Bacterial / genetics
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Gene Expression Regulation, Bacterial
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Genes, Bacterial*
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Lactams, Macrocyclic
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Models, Molecular
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Molecular Sequence Data
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Multigene Family*
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Quinones / isolation & purification
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Quinones / metabolism*
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Quinones / pharmacology
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Streptomyces / genetics*
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Streptomyces / metabolism
Substances
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Benzoquinones
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DNA, Bacterial
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Lactams, Macrocyclic
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Quinones
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geldanamycin