Abstract
Successive extracts of Tribulus terrestris prepared using petroleum ether, chloroform, 50% methanol and water were tested for anthelmintic activity in-vitro using the nematode Caenorhabditis elegans. The activity could be detected only in 50% methanol extract which on further bioactivity guided fractionation and chromatographic separation yielded a spirostanol type saponin, tribulosin and beta-sitosterol-D-glucoside. Both the compounds exhibited anthelmintic activity with ED50 of 76.25 and 82.50 microg/ml respectively.
MeSH terms
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Animals
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Antinematodal Agents / administration & dosage
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Antinematodal Agents / pharmacology*
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Antinematodal Agents / therapeutic use
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Caenorhabditis elegans / drug effects*
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Humans
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Lethal Dose 50
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Nematode Infections / drug therapy
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Phytotherapy*
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Plant Extracts / administration & dosage
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Plant Extracts / pharmacology
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Plant Extracts / therapeutic use
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Sitosterols / administration & dosage
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Sitosterols / pharmacology*
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Sitosterols / therapeutic use
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Tribulus*
Substances
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Antinematodal Agents
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Plant Extracts
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Sitosterols
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tribulosin
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lyoniside