Tribulosin and beta-sitosterol-D-glucoside, the anthelmintic principles of Tribulus terrestris

Phytomedicine. 2002 Dec;9(8):753-6. doi: 10.1078/094471102321621395.

Abstract

Successive extracts of Tribulus terrestris prepared using petroleum ether, chloroform, 50% methanol and water were tested for anthelmintic activity in-vitro using the nematode Caenorhabditis elegans. The activity could be detected only in 50% methanol extract which on further bioactivity guided fractionation and chromatographic separation yielded a spirostanol type saponin, tribulosin and beta-sitosterol-D-glucoside. Both the compounds exhibited anthelmintic activity with ED50 of 76.25 and 82.50 microg/ml respectively.

MeSH terms

  • Animals
  • Antinematodal Agents / administration & dosage
  • Antinematodal Agents / pharmacology*
  • Antinematodal Agents / therapeutic use
  • Caenorhabditis elegans / drug effects*
  • Humans
  • Lethal Dose 50
  • Nematode Infections / drug therapy
  • Phytotherapy*
  • Plant Extracts / administration & dosage
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use
  • Sitosterols / administration & dosage
  • Sitosterols / pharmacology*
  • Sitosterols / therapeutic use
  • Tribulus*

Substances

  • Antinematodal Agents
  • Plant Extracts
  • Sitosterols
  • tribulosin
  • lyoniside