Induction of human mammary-associated serum amyloid A3 expression by prolactin or lipopolysaccharide

Biochem Biophys Res Commun. 2003 Feb 21;301(4):1030-7. doi: 10.1016/s0006-291x(03)00045-7.


In most mammalian species, serum amyloid A isoform 3 (SAA3) appears to be the predominant SAA isoform expressed extrahepatically. However, human SAA3 gene expression has not been detected previously and, therefore, this gene was referred to as a pseudogene. We report for the first time the transcriptional expression of human SAA3. Human SAA3 gene expression was detected by RT-PCR after stimulation of mammary gland epithelial cells with either prolactin (PRL) or lipopolysaccharide (LPS). The full-length 655bp cDNA sequence for this mammary-associated serum amyloid A3 (M-SAA3) was obtained using 5' and 3' rapid amplification of cDNA ends (RACE). The human M-SAA3 transcript would conceptually translate into a 42 residue mature protein, which is smaller than other mammalian SAA3 isoforms that are typically 104-113 amino acids in length. This study defines the cDNA sequence for human SAA3 and also demonstrates the upregulation of M-SAA3 expression in response to the lactational hormone PRL or to an acute phase stimulant such as LPS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Breast / drug effects*
  • Breast / metabolism*
  • Cell Line
  • DNA Primers / genetics
  • DNA, Complementary / genetics
  • Epithelial Cells / drug effects
  • Epithelial Cells / metabolism
  • Female
  • Gene Expression / drug effects
  • Humans
  • Lipopolysaccharides / pharmacology*
  • Molecular Sequence Data
  • Prolactin / pharmacology*
  • Protein Isoforms / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Serum Amyloid A Protein / genetics*


  • DNA Primers
  • DNA, Complementary
  • Lipopolysaccharides
  • Protein Isoforms
  • SAA3P protein, human
  • Serum Amyloid A Protein
  • Prolactin

Associated data

  • GENBANK/AY209188