Inhibition of Epstein-Barr virus lytic cycle by (-)-epigallocatechin gallate

Biochem Biophys Res Commun. 2003 Feb 21;301(4):1062-8. doi: 10.1016/s0006-291x(03)00067-6.

Abstract

(-)-Epigallocatechin gallate (EGCG), abundant in green tea, is a potent anti-microbial and anti-tumor compound. This investigation used immunoblot, flow cytometry, microarray, and indirect immunofluorescence analyses to show that at concentrations exceeding 50 microM, EGCG inhibits the expression of Epstein-Barr virus (EBV) lytic proteins, including Rta, Zta, and EA-D, but does not affect the expression of EBNA-1. Moreover, DNA microarray and transient transfection analyses demonstrated that EGCG blocks EBV lytic cycle by inhibiting the transcription of immediate-early genes, thus inhibiting the initiation of EBV lytic cascade.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antiviral Agents / pharmacology*
  • Catechin / analogs & derivatives*
  • Catechin / pharmacology*
  • Epstein-Barr Virus Nuclear Antigens / biosynthesis
  • Epstein-Barr Virus Nuclear Antigens / genetics
  • Flow Cytometry
  • Fluorescent Antibody Technique, Indirect
  • Genes, Immediate-Early / drug effects
  • Herpesvirus 4, Human / drug effects*
  • Herpesvirus 4, Human / genetics
  • Herpesvirus 4, Human / pathogenicity
  • Herpesvirus 4, Human / physiology*
  • Humans
  • Oligonucleotide Array Sequence Analysis
  • Transcription, Genetic / drug effects
  • Transfection
  • Tumor Cells, Cultured
  • Viral Proteins / biosynthesis
  • Viral Proteins / genetics

Substances

  • Antiviral Agents
  • Epstein-Barr Virus Nuclear Antigens
  • Viral Proteins
  • Catechin
  • epigallocatechin gallate
  • EBV-encoded nuclear antigen 1