Abstract
(-)-Epigallocatechin gallate (EGCG), abundant in green tea, is a potent anti-microbial and anti-tumor compound. This investigation used immunoblot, flow cytometry, microarray, and indirect immunofluorescence analyses to show that at concentrations exceeding 50 microM, EGCG inhibits the expression of Epstein-Barr virus (EBV) lytic proteins, including Rta, Zta, and EA-D, but does not affect the expression of EBNA-1. Moreover, DNA microarray and transient transfection analyses demonstrated that EGCG blocks EBV lytic cycle by inhibiting the transcription of immediate-early genes, thus inhibiting the initiation of EBV lytic cascade.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antiviral Agents / pharmacology*
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Catechin / analogs & derivatives*
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Catechin / pharmacology*
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Epstein-Barr Virus Nuclear Antigens / biosynthesis
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Epstein-Barr Virus Nuclear Antigens / genetics
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Flow Cytometry
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Fluorescent Antibody Technique, Indirect
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Genes, Immediate-Early / drug effects
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Herpesvirus 4, Human / drug effects*
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Herpesvirus 4, Human / genetics
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Herpesvirus 4, Human / pathogenicity
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Herpesvirus 4, Human / physiology*
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Humans
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Oligonucleotide Array Sequence Analysis
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Transcription, Genetic / drug effects
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Transfection
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Tumor Cells, Cultured
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Viral Proteins / biosynthesis
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Viral Proteins / genetics
Substances
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Antiviral Agents
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Epstein-Barr Virus Nuclear Antigens
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Viral Proteins
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Catechin
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epigallocatechin gallate
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EBV-encoded nuclear antigen 1