Molecular Cloning of the Beta Subunit of Complement Component Eight of Rainbow Trout

Dev Comp Immunol. 2003 Mar;27(3):167-74. doi: 10.1016/s0145-305x(02)00092-7.

Abstract

Complement-mediated killing of pathogens through the lytic pathway is an important effector mechanism of the innate immune response. C8 is one of the components of the lytic pathway and is composed of an alpha, beta, and gamma subunit. In the present study we report the cloning and characterization of the primary structure of the C8beta subunit in the rainbow trout (Oncorhynchus mykiss). The deduced amino acid sequence of trout C8beta shows 72 and 47% identity with that of Japanese flounder and human, respectively. It also contains many of the same structural motifs as those found in mammalian lytic components. The C8beta gene appears to exists as a single copy in the trout genome and is expressed primarily in the liver. The protein encoded by the gene was identified by Western blotting using an anti-peptide antibody and was approximately 65kDa.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Southern
  • Cloning, Molecular
  • Complement C8 / chemistry
  • Complement C8 / genetics*
  • Complement C9 / chemistry
  • Molecular Sequence Data
  • Oncorhynchus mykiss / immunology*
  • Protein Subunits
  • RNA, Messenger / analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Alignment
  • Sequence Analysis, DNA

Substances

  • Complement C8
  • Complement C9
  • Protein Subunits
  • RNA, Messenger

Associated data

  • GENBANK/AF418597