Identification of human dehydrodolichyl diphosphate synthase gene

Shota Endo; Yuan-Wei Zhang; Seiji Takahashi; Tanetoshi Koyama

doi:10.1016/s0167-4781(02)00628-0

Identification of human dehydrodolichyl diphosphate synthase gene

Biochim Biophys Acta. 2003 Feb 20;1625(3):291-5. doi: 10.1016/s0167-4781(02)00628-0.

Authors

Shota Endo¹, Yuan-Wei Zhang, Seiji Takahashi, Tanetoshi Koyama

Affiliation

¹ Institute of Multidisciplinary Research for Advanced Materials (IMRAM), Tohoku University, Katahira 2-1-1, Aoba-ku, 980-8577, Sendai, Japan.

PMID: 12591616
DOI: 10.1016/s0167-4781(02)00628-0

Abstract

We isolated a cDNA encoding human dehydrodolichyl diphosphate (Dedol-PP) synthase and expressed the gene in a yeast mutant strain SNH23-7D, which is deficient in Dedol-PP synthase activity. The identity of the cloned enzyme was confirmed by functional complementation of SNH23-7D strain together with in vitro Dedol-PP synthase activity assay. Northern blot analysis indicated that testis and kidney expressed Dedol-PP synthase mRNA at high levels.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Alkyl and Aryl Transferases / analysis
Alkyl and Aryl Transferases / biosynthesis
Alkyl and Aryl Transferases / genetics*
Amino Acid Sequence
Blotting, Northern
DNA, Complementary / biosynthesis
DNA, Complementary / chemistry
Dolichols / biosynthesis
Gene Expression
Genetic Complementation Test
Humans
Kidney / enzymology
Male
Molecular Sequence Data
RNA, Messenger / analysis
Sequence Alignment
Sequence Homology, Amino Acid
Testis / enzymology
Yeasts / metabolism

Substances

DNA, Complementary
Dolichols
RNA, Messenger
Alkyl and Aryl Transferases
dehydrodolichyl diphosphate synthetase

Associated data

GENBANK/AB090852