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. 2003 Mar;185(5):1719-25.
doi: 10.1128/jb.185.5.1719-1725.2003.

DNA Microarray Analysis of Redox-Responsive Genes in the Genome of the Cyanobacterium Synechocystis Sp. Strain PCC 6803

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Free PMC article

DNA Microarray Analysis of Redox-Responsive Genes in the Genome of the Cyanobacterium Synechocystis Sp. Strain PCC 6803

Yukako Hihara et al. J Bacteriol. .
Free PMC article

Abstract

Whole-genome DNA microarrays were used to evaluate the effect of the redox state of the photosynthetic electron transport chain on gene expression in Synechocystis sp. strain PCC 6803. Two specific inhibitors of electron transport, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) and 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB), were added to the cultures, and changes in accumulation of transcripts were examined. About 140 genes were highlighted as reproducibly affected by the change in the redox state of the photosynthetic electron transport chain. It was shown that some stress-responsive genes but not photosynthetic genes were under the control of the redox state of the plastoquinone pool in Synechocystis sp. strain PCC 6803.

Figures

FIG. 1.
FIG. 1.
Decay kinetics of fluorescence in the millisecond range after excitation with a single-turnover flash. The data are normalized with variable fluorescence. Measurement were performed without inhibitors (A), in the presence of 10 μM DCMU (B), and in the presence of 10 μM DBMIB (C).
FIG. 2.
FIG. 2.
Induction or repression ratio of each gene for the inhibitors DCMU and DBMIB. Twofold induction and 50% repression are shown by solid lines. A >3-fold difference in the effects of the two inhibitors is shown by dashed lines. The four groups categorized by inhibitor effect are indicated.

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