SF4 and SFRS14, two related putative splicing factors on human chromosome 19p13.11

Gene. 2003 Feb 13;305(1):91-100. doi: 10.1016/s0378-1119(02)01230-1.

Abstract

The splicing of nascent mRNA precursors is an essential step for the expression of all intron-containing eukaryotic genes. Removal of intron sequences from nascent transcripts is mediated by the spliceosome, a large multicomponent complex. We describe here the identification of two genes encoding related, putative splicing factors on human chromosome 19p13.11, SF4 (splicing factor 4) and SFRS14 (splicing factor arginine/serine-rich 14). Both genes encode proteins containing a SURP motif; this domain is found in several splicing proteins including Drosophila alternative splicing regulator, suppressor-of-white-apricot (SWAP) and the yeast splicing factor, prp21p. In addition, SF4 and SFRS14 contain a G-patch domain at their C-termini, a motif present in a large number of eukaryotic RNA-binding proteins. SFRS14 also contains an N-terminal region that is rich in arginine/serine residues, suggesting SFRS14 is a novel member of the SR-related family of pre-mRNA processing factors. We have also identified the mouse orthologues of SF4 and SFRS14, based on conserved domain organization and high sequence similarity. Interestingly, SFRS14 undergoes alternative 3'-end processing events that are conserved between human and mouse, suggesting a functional significance.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / embryology
  • Brain / metabolism
  • Chromosomes, Human, Pair 19 / genetics*
  • Cloning, Molecular
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • Exons
  • Gene Expression
  • Gene Expression Regulation, Developmental
  • Genes / genetics
  • Humans
  • Introns
  • Male
  • Mice
  • Molecular Sequence Data
  • Nuclear Proteins
  • RNA Splicing Factors
  • RNA Splicing*
  • RNA-Binding Proteins / genetics*
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Serine-Arginine Splicing Factors

Substances

  • DNA, Complementary
  • Nuclear Proteins
  • RNA Splicing Factors
  • RNA-Binding Proteins
  • SUGP1 protein, human
  • Serine-Arginine Splicing Factors

Associated data

  • GENBANK/AF518874
  • GENBANK/AF518875
  • GENBANK/AF521128
  • GENBANK/AF521129