Sequential waves of functionally related proteins are expressed when B cells prepare for antibody secretion

Immunity. 2003 Feb;18(2):243-53. doi: 10.1016/s1074-7613(03)00024-4.


Upon encounter with antigen, B lymphocytes differentiate into Ig-secreting plasma cells. This step involves a massive development of secretory organelles, most notably the endoplasmic reticulum. To analyze the relationship between organelle reshaping and Ig secretion, we performed a dynamic proteomics study of B lymphoma cells undergoing in vitro terminal differentiation. By clustering proteins according to temporal expression patterns, it appeared that B cells anticipate their secretory role in a multistep process. Metabolic capacity and secretory machinery expand first to accommodate the mass production of IgM that follows.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B-Lymphocytes / cytology
  • B-Lymphocytes / immunology*
  • B-Lymphocytes / metabolism
  • Cell Differentiation
  • Cell Line
  • Endoplasmic Reticulum / immunology
  • Endoplasmic Reticulum / metabolism
  • Energy Metabolism
  • Gene Expression
  • Immunoglobulin M / biosynthesis*
  • Lipopolysaccharides / pharmacology
  • Mice
  • Oxidation-Reduction
  • Plasma Cells / cytology
  • Plasma Cells / immunology
  • Plasma Cells / metabolism
  • Proteins / genetics*
  • Proteomics


  • Immunoglobulin M
  • Lipopolysaccharides
  • Proteins