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. 2003 Mar;71(3):1566-8.
doi: 10.1128/iai.71.3.1566-1568.2003.

Role of interleukin-6 in the Control of Acute and Chronic Giardia Lamblia Infections in Mice

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Free PMC article

Role of interleukin-6 in the Control of Acute and Chronic Giardia Lamblia Infections in Mice

Ping Zhou et al. Infect Immun. .
Free PMC article

Abstract

In this study, we investigated the role of interleukin-6 (IL-6) in Giardia lamblia infections in mice. Elevated IL-6 expression was found in wild-type mice 15 days postinfection. Furthermore, IL-6-deficient mice controlled infections only slowly although normal immunoglobulin A production was observed. Thus, IL-6 is necessary for early control of acute G. lamblia infections.

Figures

FIG. 1.
FIG. 1.
IL-6 mRNA expression in infected C57BL/6J mice. RNA was prepared from the small intestines of wild-type mice infected for 0, 5, or 15 days with G. lamblia (Fig. 2). IL-6 and HPRT mRNA levels were assayed by RT-PCR. The plus sign represents amplification controls with pGEM-IL-6Δ (IL-6) or pLOC21 (HPRT). Since these plasmids contained competitor templates, sizes are somewhat different than the PCR product from cDNA. The minus sign indicates no template controls for PCR. Data are representative of three individual experiments totaling at least 12 mice per time point.
FIG. 2.
FIG. 2.
Defective control of acute infections in IL-6-deficient mice. Wild-type and IL-6-deficient mice were infected on day 0, and parasite loads were determined 5, 15, 28, and 60 days postinfection as described previously (15). *, P < 0.05. nd, none detected. Results are representative of three different experiments.
FIG. 3.
FIG. 3.
IgA responses to G. lamblia infections in wild-type and IL-6-deficient mice. Intestinal washes diluted 1:5 were used to stain in vitro cultures of trophozoites. Panels on the left are from wild-type mice, and those on the right are from IL-6-deficient mice. Early IgA responses reacted with variant-specific epitopes, while at day 60, responses to invariant epitopes and/or antigens became apparent. Data are representative of at least four mice per time point per genotype.

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