Ribonuclease is partly responsible for the HIV-1 inhibitory effect activated by HLA alloantigen recognition

AIDS. 2003 Mar 7;17(4):481-6. doi: 10.1097/00002030-200303070-00002.


Objective: This study was performed to determine whether ribonucleases (RNases) contribute to the soluble HIV-1 inhibitory activity that results from the recognition of HLA alloantigens.

Design and methods: Supernatants from mixed lymphocyte reactions of peripheral blood mononuclear cells from healthy HLA-discordant individuals exhibited HIV-1 inhibitory activity (alloantigen-stimulated factors; ASF). These supernatants were tested for their sensitivity to heating (90 degrees C for 3 min), and for the presence of three RNases belonging to the RNase A superfamily: eosinophil-derived neurotoxin (EDN); RNase A; and angiogenin. Polyclonal antibodies specific for these RNases were used for Western blot analysis of the ASF, as well as for blocking the HIV-1 inhibitory activity of ASF. In addition, an RNase inhibitor (RI) was used to determine whether the anti-viral activity of ASF was due to RNase activity.

Results: HIV-1 inhibitory activity of ASF was: (i). resistant to heat treatment; (ii). blocked by 58% with an antibody specific for EDN, but not with antibodies against RNase A or angiogenin; and (iii) blocked by 65-100% with an RI. Moreover, Western blot analysis with an anti-EDN antibody detected EDN in the ASF.

Conclusion: These findings indicate that the majority of the soluble HIV-1 inhibitory activity contained in the supernatants of mixed lymphocyte reactions is due to EDN or a closely related RNase.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antiviral Agents*
  • HIV Infections / immunology*
  • HIV-1*
  • Histocompatibility Antigens / immunology*
  • Humans
  • Immunity, Innate
  • Lymphocyte Activation
  • Lymphocyte Culture Test, Mixed
  • Ribonucleases / metabolism*


  • Antiviral Agents
  • Histocompatibility Antigens
  • Ribonucleases