Regulating access to the genome: nucleocytoplasmic transport throughout the cell cycle

Cell. 2003 Feb 21;112(4):441-51. doi: 10.1016/s0092-8674(03)00082-5.


Macromolecular transport between the cytoplasm and the nucleus occurs through the nuclear pore complex (NPC) and is mediated by multiple families of soluble transport factors. All these transport factors share the ability to translocate across the NPC through specific interactions with components of the nuclear pore. This review highlights advances in our understanding of the structure and function of the NPC and the shuttling transport receptors involved in nuclear transport. It discusses recently proposed models for the translocation of receptor-cargo complexes through the NPC channel and reviews how the small GTPase Ran functions as a positional marker of the genome to regulate multiple important aspects of the eukaryotic cell cycle.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Active Transport, Cell Nucleus*
  • Amino Acid Motifs
  • Animals
  • Cell Cycle
  • Cell Nucleus / metabolism
  • Cell Nucleus / ultrastructure*
  • Chromatin / metabolism
  • Cytoplasm / metabolism*
  • Genome*
  • Genome, Fungal
  • Interphase
  • Karyopherins / metabolism
  • Mitosis
  • Models, Biological
  • Protein Structure, Secondary
  • Structure-Activity Relationship
  • beta Karyopherins / metabolism
  • ran GTP-Binding Protein / metabolism


  • Chromatin
  • Karyopherins
  • beta Karyopherins
  • ran GTP-Binding Protein