The ability to see fine detail diminishes when the target of interest moves at a speed greater than a few deg/s. The purpose of this study was to identify fundamental limitations on spatial acuity that result from image motion. Discrimination of Vernier offset was measured for a pair of vertical abutting lines and letter resolution was measured using a four-orientation letter 'T'. These stimuli were digitally filtered using one of five band-pass (bandwidth=1.5 octaves) filters with a center frequency between 0.83 and 13.2 c/deg, and presented at velocities that ranged from 0 to 12 deg/s. Filtered and unfiltered stimuli were presented for 150 ms at a constant multiple (4x or 2x) of the contrast-detection threshold at each velocity. For stimuli of low to middle spatial frequency (up to 3.3 c/deg), Vernier and letter acuity for equally detectable targets are essentially unaffected by velocity up to 12 deg/s, i.e., for temporal frequencies of motion (velocity x spatial frequency) up to approximately 50 Hz. For stimuli of higher spatial frequency, acuity remains essentially constant until the velocity corresponds to a temporal frequency of about 30 Hz, and increases thereafter. Both Vernier and letter acuities worsen by approximately a factor of two for each one-octave decrease in filter spatial frequency. Both types of acuities worsen also as the contrast of the stimulus is reduced, but Vernier discrimination exhibits a stronger contrast-dependence than letter resolution. Our results support previous suggestions that a shift in the spatial scale used by the visual system to analyze spatial stimuli is principally responsible for the degradation of acuity in the presence of image motion. The results are consistent with a spatio-temporal-frequency limitation on spatial thresholds for moving stimuli, and not with a temporal-frequency limitation per se.