Over-expression of tenascin-C in malignant pleural mesothelioma

Histopathology. 2003 Mar;42(3):280-91. doi: 10.1046/j.1365-2559.2003.01568.x.


Aims: Tenascin-C is an extracellular matrix glycoprotein known to have anti-adhesive characteristics and to be expressed in various human malignant neoplasms. We hypothesized that the expression of tenascin-C would be increased in human malignant pleural mesothelioma, and its accumulation associated with the prognosis of the patients with this disease.

Methods and results: Thirty-seven cases of mesothelioma were studied by immunohistochemically using a monoclonal antibody against tenascin-C, and with a semiquantitative scoring system for tenascin-C in different areas of the tumours. In 10 selected cases tenascin-C mRNA in-situ hybridization was also analysed. Since transforming growth factor-beta (TGF-beta) is known to induce both the synthesis of tenascin-C and the growth of mesotheliomas, an immunohistochemical analysis of TGF-beta 1, -beta 2 and -beta 3 was also performed. Normal pleura (n = 7) and metastatic pleural adenocarcinomas (n = 7) were used as controls. Tenascin-C protein was expressed in every histological subtype of malignant mesothelioma, being most prominent in the fibrotic stroma of a tumour, around tumour cells and at the invasive border, whereas tenascin-C mRNA was scarce in tumour cells. The patients with less immunohistochemical expression for tenascin-C tended to live longer (P = 0.028 by Fishers' exact probability test). All mesotheliomas showed positivity for at least one isoform of TGF-beta.

Conclusions: In conclusion, high expression of tenascin-C protein in malignant pleural mesotheliomas may play a role in its invasive growth, and might serve as a prognostic marker of the disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / genetics
  • Adenocarcinoma / metabolism
  • Adenocarcinoma / secondary
  • Adult
  • Aged
  • Biomarkers, Tumor / metabolism
  • Female
  • Humans
  • Immunoenzyme Techniques
  • In Situ Hybridization
  • Male
  • Mesothelioma / genetics
  • Mesothelioma / metabolism*
  • Mesothelioma / pathology
  • Middle Aged
  • Neoplasm Proteins / metabolism
  • Pleural Neoplasms / genetics
  • Pleural Neoplasms / metabolism*
  • Pleural Neoplasms / pathology
  • Prognosis
  • RNA, Messenger / metabolism
  • RNA, Neoplasm / analysis
  • Tenascin / genetics
  • Tenascin / metabolism*
  • Transforming Growth Factor beta / metabolism


  • Biomarkers, Tumor
  • Neoplasm Proteins
  • RNA, Messenger
  • RNA, Neoplasm
  • Tenascin
  • Transforming Growth Factor beta