Tissue-specific expression, heat inducibility, and biological roles of two hsp16 genes in Caenorhabditis elegans

FEBS Lett. 2003 Feb 27;537(1-3):139-45. doi: 10.1016/s0014-5793(03)00111-x.


In this report we have examined two new heat shock protein (HSP16) proteins in the nematode Caenorhabditis elegans encoded by the open reading frames F08H9.3 and F08H9.4. The F08H9.3 and F08H9.4 genes are oriented in the same direction next to each other on the chromosome, not sharing any promoter region, unlike other hsp16 genes that share common promoters in pairs. The F08H9.3 and F08H9.4 proteins were expressed in a tissue-specific manner, unlike the other four HSP16 proteins. F08H9.3 was expressed in the pharynx, and F08H9.4 in the excretory canal and a few neuronal cells. While F08H9.3 was weakly induced by heat shock only in the same tissue as under the normal condition, F08H9.4 was newly induced in the intestine. RNA interference experiments showed that these two proteins are required for survival under the heat shock condition.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Caenorhabditis elegans / genetics
  • Caenorhabditis elegans / physiology*
  • Caenorhabditis elegans Proteins / genetics*
  • DNA Primers
  • Gene Expression Regulation*
  • Heat-Shock Proteins / chemistry
  • Heat-Shock Proteins / genetics*
  • Heat-Shock Proteins / physiology
  • Hot Temperature
  • Molecular Sequence Data
  • Multigene Family
  • Organ Specificity
  • Promoter Regions, Genetic
  • Protein Isoforms / chemistry
  • Protein Isoforms / genetics
  • Protein Isoforms / physiology
  • Sequence Alignment
  • Sequence Homology, Amino Acid


  • Caenorhabditis elegans Proteins
  • DNA Primers
  • F08H9.3 protein, C elegans
  • F08H9.4 protein, C elegans
  • Heat-Shock Proteins
  • Protein Isoforms

Associated data

  • GENBANK/AC006774
  • GENBANK/U64837
  • GENBANK/Z77657